Intended Use : The canine parvo virus antigen rapid test is a lateral flow immunochromatography assay for the qualitative detection of canine parvo virus antigen ( CPV test in dogs feces or vomit specimen.
Assay Time Duration : 5 – 10 minutes
The canine parvo virus antigen rapid test is based on sandwich lateral flow immunochromatography assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T ( test ) zone and a C ( control ) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre – coated monoclonal antibodies. If there is CPV antigen in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By the means, the device can accurately indicate the presence of Parvo virus antigen in the specimen.
Reagent And Material Used :
1. Test devices, with disposable droppers.
2. Assay buffer.
3. Cotton swabs.
4. Products Manual.
Storage And Stability :
This kit can be stored at room temperature ( 4 – 30 degree C ). The test kit is stable through the expiration date ( 24 months ) marked on the package label. Do not freeze. Do not store the test kit in direct sunlight.
Procedure : How to do this test ?
- Firstly you have to collect the fresh feces or the vomit with the cotton swab from the dogs anus or from the ground.
- Insert the test into the provided assay buffer tube. Finally agitates it to get efficient sample extraction.
- Take out the test device from the foil pouch and place it horizontally.
- Suck the treated sample extraction from the assay buffer tube and place 3 drops into the “S” of the test device.
- Interpret the result in 5 – 10 minutes. Result after the 10 minutes is considered as invalid.
Interpretation of results :
Positive ( + ) : The presence of both “C” line and zone “T” line, no matter T line is clear or vague.
Negative ( – ) : Only clear C line appear. No T line.
Invalid : No colored line appears in C zone. No matter if T line appears.
Precautions :
- All reagents must be at room temperature before running the assay.
- Do not remove test cassette from its pouch until immediately before use.
- Do not use the test beyond its expiration date.
- The components in this kit have been quality control tested as standard batch unit. Do not mix the components from the different lot numbers.
- All specimens are of potential infection. It must be strictly treated according to the rules and regulations by local states.
Limitations :
Canine Parvo Virus Antigen Rapid Test is for in vitro veterinary diagnosis use only. All the results should be considered with the the other clinical information available with the veterinarian. It is suggested to apply a further confirmative method when positive result was observed.